Funded: 2016

Fellow: 
Dr. Liying Jiang; King’s College London Drug Control Centre

Mentor: 
Dr. David Cowan; King’s College London Drug Control Centre

Research Title:

Strategy for cost reduction in doping test using UPLC-ESPI-TOFMS for urine and DESI-QTOFMS for DBS

Following completion of his fellowship, Dr. Geoff Miller accepted a full time position at SMRTL as Research & Laboratory Manager.

Funded: 2014

Fellow:
Dr. Geoff Miller; Sports Medicine Research and Testing Laboratory (SMRTL)

Mentor:
Dr. Daniel Eichner; Sports Medicine Research and Testing Laboratory (SMRTL)

Research Title:

Assessing hydration status through evaluation of albumin osmolality and lactate for the ABP

Publications:

American Academy of Physical Medicine and Rehabilitation: The Anti-Doping Movement

Drug Testing and Analysis: Intranasal delivery of Natesto testosterone gel and its effects on doping markers

Presentations: 

Miller G, Teramoto M, Cushman D, Eichner D. Assessing Dehydration in the Context of the
Biological Passport. 34th Cologne Workshop on Dope Analysis; Cologne, Germany. February 2016.

Nair V, Miller G, Morrison S, Summers M, Willick S, Eichner D. Intranasal Delivery of Natesto
Testosterone Gel and Its Effects on Doping Markers. 34th Cologne Workshop on Dope Analysis;
Cologne, Germany. February 2016.

Miller, G. The next generation of anti-doping: PCC Fellowship. Partnership for Clean Competition Conference; New York, NY, April 2017.

Funded: 2012

Fellow:
Dr. Bryan Kelly; Sports Medicine Research and Testing Laboratory (SMRTL)

Mentor:
Dr. Daniel Eichner; Sports Medicine Research and Testing Laboratory (SMRTL)

Funded: 2016

Principle Investigator:
Dr. Mario Thevis; German Sport University Cologne

Publication: 

Rapid Communications in Mass Spectrometry: Expanding Analytical Options in Sports Drug Testing: Mass Spectrometric Detection of Prohibited Substances in Exhaled Breath

Funded: 2016

Principle Investigator:
Dr. Mario Thevis; German Sport University Cologne

Publications:

International Journal of Sports Medicine: Analyses of Meldonium (Mildronate) from Blood, Dried Blood Spots (DBS), and urine Suggest Drug Incorporation into Erythrocytes.

Analytical and Bioanalytical Chemistry: Qualitative identification of growth hormone-releasing hormones in human plasma by means of immunoaffinity purification and LC-HRMS/MS

Funded: 2014

Principle Investigator:
Dr. Pauline Rudd; National Institute for Bioprocessing Research and Training (NIBRT)

Funded: 2014

Principle Investigator:
Dr. Mario Thevis; German Sport University Cologne

Publications:
Drug Testing & Analysis: Mildronate (Meldonium) in professional sports – monitoring doping control urine samples using hydrophilic interaction liquid chromatography – high resolution/high accuracy mass spectrometry

International Journal of Sports Medicine: Analyses of Meldonium (Mildronate) from Blood, Dried Blood Spots (DBS), and Urine Suggest Drug Incorporation into Erythrocytes

Funded: 2014

Principle Investigator:
Dr. Daniel Eichner; Sports Medicine Research and Testing Laboratory (SMRTL)

Funded: 2016

Principle Investigator:
Dr. Daniel Eichner; Sports Medicine Research and Testing Laboratory (SMRTL)

Complete

Funded: 2016

Principle Investigator:
Professor David Handelsman; ANZAC Research Institute

Publication: 

JCEM: Urine and Serum Sex Steroid Profile in Testosterone-Treated Transgender and Hypogonadal and Healthy Control Men.

Funded: 2016

Principle Investigator:
Dr. Daniel Eichner; Sports Medicine Research and Testing Laboratory (SMRTL)

Presentations: 

Cox, HD, Eichner, D. Targeted quantification of blood cell marker proteins in dried blood spots for the detection of blood doping practices in sport. June 2015. 63rd Meeting of the American Society for Mass Spectrometry. St. Louis, MO.

Cox, HD, Eichner, D. Preliminary evaluation of a novel dried blood spot-based methodology for the detection of blood doping practices in sport. September 2014. USADA Science Symposium. Phoenix, AZ.

Cox, HD, Hughes, CM, Eichner, D. Method to detect blood doping practices in sport through the measurement of reticulocytes in dried blood spots by LC-MS/MS. June 2014. 62nd Meeting of the American Society for Mass Spectrometry. Baltimare, MD.

Funded: 2016

Principle Investigator:
Dr. Lena Ekstrom; Karolinska Instituet 

Presentations: 

Estrom, L. Testosterone Codeine Study. 35th Manfred Donike Workshop on Dope Analysis. 2017, Cologne, Germany.

Funded: 2016

Principle Investigator:
Prof. Dr. Maria Kristina Parr; Freie Universitat Berlin

Funded: 2016

Principle Investigator:
Dr. Jack Henion; Q2 Solutions

Presentations:

Henion, J.  Do DBS and DPS Micro Sampling Techniques have a place in the Clinical Laboratory? MSACL 2016 9^th Annual Conference & Exhibits, Jan 22 – 26, Palm Springs, CA, 2017

Henion, J. The Analytical Potential of a Compact Mass Spectrometer (CMS) for the Analysis of Cannabis-Related Samples for Composition and Adulteration. Pittcon Conference and Expo in Chicago, Illinois, 2017 March 5 – 9 .

Henion, J. Alternative Matrices: Dried Plasma Spots. Partnership for Clean Competition Conference. New York, NY, 2017

Funded: 2017

Principle Investigator:
Dr. Robert Chapman; Indiana University

Funded: 2016

Principle Investigator:
Dr. Herb Tobias; Dell Pediatric Research Institute

Funded: 2017

Principle Investigator:
Dr. Pozo Mendoza; IMIM: Institut Hospital del Mar d’Investigacions Mèdiques

Funded: 2017

Principle Investigator:
Dr. Mariana Renovato Martins;  Instituto Federal do Rio de Janeiro

Funded: 2017

Principle Investigator:
Dr. Robert Chapman, Indiana University 

Principle Investigators:
Dr. Mario Thevis; German Sport University Cologne
Dr. Daniel Eichner; Sports Medicine Research and Testing Laboratory (SMRTL)
Dr. David Cowan; King’s College London Drug Control Centre
Dr. Anthony Butch; UCLA Olympic Analytical Laboratory
Dr. Andy Hoofnagle; University of Washington

Publications:

Clinical Chemistry: Interlaboratory agreement of insulin-like growth factor 1 concentrations measured by mass spectrometry

Bioanalysis: Sensitive quantification of IGF-1 and its synthetic analogs in dried blood spots

Drug Testing & Analysis: The development of decision limits for the GH-2000 detection methodology using additional insulin-like growth factor-I and amino-terminal pro-peptide of type III collagen assays

Clinical Chemistry: Immunoextraction-Tandem Mass Spectrometry Method for Measuring Intact Human Chorionic Gonadotropin, Free β-Subunit, and β-Subunit Core Fragment in Urine

Principle Investigators:
Dr. Marilyn Huestis; National Institute on Drug Abuse (NIH)
Dr. Daniel Eichner; Sports Medicine Research and Testing Laboratory (SMRTL)

Principle Investigator:
Dr. Marilyn Huestis; National Institute on Drug Abuse (NIH)

Publications: 

Drug & Alchohol Dependence: Pharmacodynamic effects and relationships to plasma and oral fluid pharmacokinetics after intravenous cocaine administration.

Drug Testing & Analysis: Cocaine and benzoylecgonine oral fluid on-site screening and confirmation.

Forensic Science International: Oral fluid cocaine and benzoylecgonine concentrations following controlled intravenous cocaine administration.

Principle Investigator:
Dr. John Higgins; Massachusetts General Hospital 

Principle Investigators:

Dr. Daniel Eichner; Sports Medicine Research and Testing Laboratory (SMRTL)
Dr. Anthony Butch; UCLA Olympic Analytical Laboratory

Lab Managers can apply for COE grants via regular PCC grant processes.

Principle Investigators:

Dr. Daniel Eichner; Sports Medicine Research and Testing Laboratory (SMRTL)
Dr. Anthony Butch; UCLA Olympic Analytical Laboratory

Lab Managers can apply for COE grants via regular PCC grant processes.

Principle Investigators:

Dr. Daniel Eichner; Sports Medicine Research and Testing Laboratory (SMRTL)
Dr. Anthony Butch; UCLA Olympic Analytical Laboratory

Lab Managers can apply for COE grants via regular PCC grant processes.

Principle Investigator:
Dr. Raul Nicoli; Swiss Anti-Doping Laboratory

Principle Investigator:
Dr. Zach Gagnon; John Hopkins University

Principle Investigator:
Dr. Jentsan Chi; Duke University

Principle Investigator:
Dr. Nicholas Turner; The Open University

Principle Investigator:
Dr. Corinne Buisson; AFLD – Agence française de lutte contre le dopage

Principle Investigator:
Dr. Daniel Eichner; Sports Medicine Research and Testing Laboratory (SMRTL)

Principle Investigator:
Dr. Daniel Eichner; Sports Medicine Research and Testing Laboratory (SMRTL)

Principle Investigator:
Dr. Jentsan Chi; Duke University

Media: 
https://cleancompetition.org/2018/04/19/just-funded-dr-chi/

Principle Investigator:
Dr. Christiane Ayotte; INRS Santé

Principle Investigator:
Dr. Paul Armishaw; National Measurement Institute

Principle Investigator:
Dr. Nicolas Voelcker; Monash University 

Principle Investigator:
Dr. James Harynuk; University of Alberta
Dr. Fred West; University of Alberta

Principle Investigator:
Dr. Emad Kiriakous; Queensland University

Principle Investigator:
Dr. Fred Schaufele; Xcell Assay

Principle Investigator:
Dr. Gaetano Cairo; University of Milan

Principle Investigator:
Dr. Christiane Ayotte; INRS Santé

Principle Investigator:
Dr. Judith Su; University of Arizona

Principle Investigator:
Dr. Nikolai Nordsborg; University of Copenhagen

Principle Investigator:
Dr. Mario Thevis; German Sport University Cologne 

Principle Investigator:
Dr. Herb Tobias; Dell Pediatric Research Institute

Principle Investigator:
Dr. Jack Henion; Q2 Solutions

Publications:

Analytical Chemistry: A Book-Type Dried Plasma Spot Card for Automated Flow-Through Elution Coupled with Online SPE-LC-MS/MS Bioanalysis of Opioids and Stimulants in blood

Analytical Chemistry: Hematocrit-Independent Quantitation of Stimulants in Dried Blood Spots: Pipet versus Microfluidic-Based Volumetric Sampling Coupled with Automated Flow-Through Desorption and Online Solid Phase Extraction-LC-MS/MS Bioanalysis.

Drug Testing and Analysis: Quantitative determination of opioids in whole blood using fully automated dried blood spot desorption coupled to on-line SPE-LC-MS/MS.

Bioanalysis: Novel membrane devices and their potential utility in blood sample collection prior to analysis of dried plasma spots

Presentations: 

Henion, J, Ryona, I. New Microsampling Hematocrit-Compatible Dried Plasma Card for Fully Automated On-Line DBSA-SPE-LC/MS/MS Bioanalysis of Opioids in Blood. Monday 6 June 2016 in San Antonio, TX on the first day of ASMS

Henion, J. Alternative Matrices: Dried Plasma Spots. April 11, 2017. Partnership for Clean Competition Conference. New York, NY.

Principle Investigators:
Dr. Peter Van Eenoo; University of Ghent Doping Control Laboratory 
Dr. Michael Polet; University of Ghent

Publications:

Journal of Chromatography: Improved sensitivity by use of gas chromatography-positive chemical ionization trip quadrupole mass spectrometry for the analysis of drug related substances. 

Rapid Communications in Mass Spectrometry: Gas chromatography/chemical ionization triple quadrupole mass spectrometry analysis of anabolic steroids: ionization and collision-induced dissociation behavior.

Journal of Mass Spectrometry: Efficient approach for the detection and identification of new androgenic metabolites by applying SRM GC-CI-MS/MS: a methandienone case study.

Presentations: 

Improved sensitivity by using chemical ionization GC-MS/MS M. Polet, W. Van Gansbeke, F. Hooghe and P. Van Eenoo 33rd Cologne workshop on Dope Analysis 01/03/2015 – 06/03/2015

Chemical ionization GC-MS/MS analysis of anabolic androgenic steroids M. Polet, W. Van Gansbeke and P. Van Eenoo 34th Cologne workshop on Dope Analysis 21/02/2016 – 26/02/2016

Chemical ionization GC-MS/MS analysis of anabolic androgenic steroids M. Polet, W. Van Gansbeke and P. Van Eenoo Agilent Nordic Scientific Forum 2016 (Gothenburg) 10/03/2016 – 11/03/2016

Principle Investigator:
Dr. Herbert Tobias; Cornell University

Presentation: 

Tobias, H.J.; Brenna, J.T. ” Cryogenic-Focusing Fast Gas Chromatography Isotope Ratio Mass Spectrometry,” 64nd ASMS Conference on Mass Spectrometry and Allied Topics, San Antonio TX, June 1-June 5, 2016.

Publications:

Manuscript in Progress

Principle Investigator:
Dr. Eleftherios Diamandis; Mount Sinai Hospital, Toronto

Publications:

Clinical Chemistry: Prostate-Specific Antigen as a Marker of Hyperandrogenism in Women and Its Implications for Antidoping

Clinical Chemistry and Laboratory Medicine: Effect of age on serum prostate-specific antigen in women.

Clinical Chemistry and Laboratory Medicine: Serum complexed and free prostate-specific antigen (PSA) for the diagnosis of the polycystic ovarian syndrome (PCOS)

Sports Medicine: Serum complexed and free prostate specific antigen levels are lower in female elite athletes in comparison to control women.   [submitted for publication].

Principle Investigator:
Dr. Paul Gregorevic; Baker IDI and Diabetes Institute

Principle Investigator:
Dr. Laura Garvican-Lewis; Australian Institute of Sport

Principle Investigator:
Dr. Nikolai Nordsborg; University of Copenhagen

Publications:

Drug Testing & Analysis: New insights for identification of doping with recombinant human erythropoietin micro-doses after high hydration

Scandanavian Journal of Science and Medicine in Sports: Acute hyperhydration reduces athlete biological passport OFF-hr score

Principle Investigator:
Dr. Paul Armishaw; National Measurement Institute

Principle Investigator:
Dr. Stephen Davies; National Measurement Institute

Principle Investigator:
Dr. Daniel Eichner; Sports Medicine Research and Testing Laboratory (SMRTL)

Principle Investigator:
Dr. Lore Geldof; University of Ghent

Presentations: 

Geldof L, Ryckx A, Verelst C, Deventer K and Van Eenoo P. Preventive anti-doping research of small molecule erythropoiesis stimulating agents: HIF stabilizers. 35th Manfred Donike Workshop on Dope Analysis. 2017. Cologne, Germany.

Publications: 

Biomedical Chromatography: In vitro and in vivo metabolism studies of dimethazine

Principle Investigator:
Dr. Chris Harrison; San Diego State University

Presentations: 

Harrison, C. Next Generation Technology for Anti-Doping: Electrophoretic Separations. 2017.  2017 Partnership for Clean Competition Conference. New York, NY.

Principle Investigator:
Dr. Christian Reichel; Seibersdorf Labor GmbH Doping Control Laboratory

Principle Investigator:
Dr. Richard Yost; University of Florida

Presentation: 

Allison J. Levy¹, Christopher D. Chouinard,¹ Robin H. J. Kemperman,¹ Nicholas R. Oranzi,¹ Richard A. Yost¹ . Improved Separation and Detection of Exogenous and Endogenous Isomeric Steroids Using Ion Mobility-Mass Spectrometry. 2017. American Society for Mass Spectrometry Annual Conference. Indianapolis, IN.

Principle Investigator:
Dr. Daniel Eichner; Sports Medicine Research and Testing Laboratory (SMRTL)

Publications:
Drug Testing and Analysis: Intranasal delivery of Natesto testosterone gel and its effects on doping markers

Principle Investigator:
Dr. Nicolas Voelcker; Monash University, Australia

Principle Investigator:
Dr. Jack Henion; Q2 Solutions

Publications:

Drug Testing and Analysis: Quantitative determination of opioids in whole blood using fully automated dried blood spot desorption coupled to on-line SPE-LC-MS/MS.

Principle Investigator:
Dr. Kara Lynch; University of California San Francisco 

Publications:

Analytical Toxicology: Optimization and Validation of High-Resolution Mass Spectrometry Data Analysis Parameters

Clinical Chemistry: Comparison of Information-Dependent Acquisition on a Tandem Quadrupole TOF vs a Triple Quadrupole Linear Ion Trap Mass Spectrometer for Broad-Spectrum Drug Screening

Presentations: 

Mass Spectrometry: Applications to the Clinical Lab Conference, San Diego, CA. “Broad Spectrum Screening Using Liquid Chromatography Quadrupole TOF MS: How Valuable is Retention Time for Identifying Compounds.” 03/2015

Mass Spectrometry: Applications to the Clinical Lab Conference, San Diego, CA. “Comparing TOF& QTOF for Comprehensive Drug Screening: Do You Really Need Fragmentation Information.” 03/2015

International Association of Therapeutic Drug Monitoring and Clinical Toxicology 14th International Congress, Rotterdam, Netherlands. “Optimization of data analysis parameters for clinical toxicology testing using high resolution mass spectrometry” 10/2015

Mass Spectrometry: Applications to the Clinical Laboratory Conference, Palm Springs, CA. “High Resolution Mass Spectrometry for Suspect Screening of Novel Psychoactive Substances: Is it a Viable Solution for Laboratories.” 02/2016

Mass Spectrometry: Applications to the Clinical Laboratory Conference, Palm Springs, CA. “New Library Search Algorithm Improves Identification of Drugs by LC-QqTOF” 02/2016

Yale University, Department of Laboratory Medicine, Research Conference Series. “Analytical Advances in Clinical Toxicology using High Resolution Mass Spectrometry.” 04/2016

Analytical World Conference/Society of Toxicological and Forensic Chemistry, Munich, Germany. “Q-TRAP or Q-TOF for comprehensive drug screening?” 05/2016

Principle Investigator:
Dr. Benjamin Levine; UT Southwestern Medical Center

Presentations: 

Lawley JS, Gatterer H. Howden EJ, Sarma S, Hearon C, Hieda M, Hendrix M, Piper T, Thevis M, Levine BD. Xenon: The Good, the Bad and the Doping”. Poster presented, Int’l Hypoxia Symposium, 2/9/17.

Principle Investigators:
Sports Medicine Research and Testing Laboratory (SMRTL) and UCLA Olympic Analytical Laboratory WADA Accredited Labs

Principle Investigator:
Dr. Mike Ashenden; Science and Industry Against Blood doping (SIAB)

Publications:

Medicine and Science in Sports: Acute hyperhydration reduces athlete biological passport OFF-hr score

Principle Investigator:
Dr. Richard Holt; University of Southampton

Publications:

Drug Testing and Analysis: Novel markers to detect recombinant human insulin-like growth factor-I (rhIGF-I)/rhIGF binding protein-3 (rhIGFBP-3) misuse in athletes

Drug Testing and Analysis: Biochemical markers of recombinant human insulin-like growth factor-I (rhIGF-I)/rhIGF binding protein-3 (rhIGFBP-3) misuse in athletes.

BMC Medical Research Methodology: Statistical methodology for age-adjustment of the GH-2000 score detecting growth hormone misuse

Indian Journal of Endocrinology and Metabolism: Detecting growth hormone misuse in athletes

Principle Investigator:
Dr. David Chen; University of British Columbia

Principle Investigator:
Dr. Daniel Eichner; Sports Medicine Research and Testing Laboratory (SMRTL)

Principle Investigator:
Dr. Daniel Eichner; Sports Medicine Research and Testing Laboratory (SMRTL)

Principle Investigator:
Dr. Peter Van Eenoo; Doping Control Laboratory, University of Ghent

Publications:

International Journal of Molecular Sciences: In vitro metabolic studies of REV-ERB agonists SR9009 and SR9011

Presentations: 

Geldof L, Deventer K, Tudela E and Van Eenoo P. In vitro metabolism studies of REV-ERBα agonists SR9009 and SR9011. 34th Manfred Donike Workshop on Dope Analysis. 2016. Cologne, Germany.

Principle Investigator:
Dr. David Cowan; King’s College London Drug Control Centre

Presentations: 

Analytical and Environmental Sciences Division Symposium, King’s College London:

1. 2015- Poster Presentation: Towards quantifying PIIINP in human serum by LC-MS: identifying suitable surrogate peptides
2. 2016- Poster Presentation: Towards quantifying PIIINP in human serum by LC-MS: separating PIIINP from human serum albumin

Principle Investigator:
Dr. Mario Thevis; German Sport University Cologne

Publications:

Analytical & Bioanalytical Chemistry: Qualitative identification of growth hormone-releasing hormones in human plasma by means of immunoaffinity purification and LC-HRMS/MS

Presentations: 

Qualitative identification of growth hormone-releasing hormones in human plasma by means of immunoaffinity purification and LC-HRMS/MS. Thevis, M. 2016. 34th Cologne workshop on Dope Analysis. Cologne, Germany.

Principle Investigator:
Dr. Pauline Rudd; NIBRT

Principle Investigator:
Dr. Emad Kiriakous; Queensland University

Publications:

Nanomedicine: Rapid isolation and detection of erythropoietin in blood plasma by magnetic core gold nanoparticles and portable Raman spectroscopy

Journal of Nanobiotechnology: Reproducible and label-free biosensor for the selective extraction and rapid detection of proteins in biological fluids

Talanta: Ultra sensitive label free surface enhanced Raman spectroscopy method for the detection of biomolecules.

Queensland University of Technology: A Novel identification method for ultra trace detection of biomolecules using functionalized-surface enhanced Raman Spectroscopy (SERS) 

Presentations: 

Hughes, Juanita, Izake, Emad L., Lott, William B.,  & Ayoko, Godwin A. (2014) Label-free SERS for natural product provenance. In 24th Anniversary World Congress on Biosensors, 28-30 May 2014, Melbourne Convention and Exhibition Centre, Melbourne, VIC.

Hughes, Juanita, Lott, William B., Ayoko, Godwin A., Sillence, Martin,  & Izake, Emad L. (2013) Ultra-trace detection of diagnostically important biomarkers using functionalised-Surface Enhanced Raman Spectroscopy (SERS). In Eighth ISABS Conference on Forensic, Anthropologic and Medical Genetics and Mayo Clinic Lectures in Translational Medicine, 24-28 June 2013, Split, Croatia.

Principle Investigator:
Dr. Ruprecht Keller; Cologne Central Hospital Lab.

Publications:

Sports Medicine: The Urine Marker Test: An Alternative Approach to Supervised Urine Collection for Doping Control

Principle Investigator:
Dr. Rodrigo Aguilera

Presentation:

Alexandra Goncalves, Beatriz Salema, João Ruivo, Sandra Ramos,  Susana Luz, Thomas Chapman and Rodrigo Aguilera. “Development and validation of a methodology for the detection of synthetic forms of Endogenous Anabolic Androgenic Steroids in urine samples by GC-C-IRMS.”XVI Latino-Americano Conference on Chromatography. Lisbon, 5 to 9 January 2016

Principle Investigator:
Dr. Anthony Butch; UCLA Olympic Analytical Laboratory

Presentations: 

Tu, M., Chia, D., Wei, F., Wong, D., and Butch, A. Amperometric Immunosensor for Detecting Growth Hormone Use by Athletes. 15th Annual UC Systemwide Bioengineering Symposium. June 2014, California, USA.

Principle Investigator:
Dr. David Cowan; King’s College London Drug Control Centre

Presentations: 

Analytical and Environmental Sciences Division Symposium, King’s College London:

1. 2015- Poster Presentation: Towards quantifying PIIINP in human serum by LC-MS: identifying suitable surrogate peptides
2. 2016- Poster Presentation: Towards quantifying PIIINP in human serum by LC-MS: separating PIIINP from human serum albumin

Principle Investigator:
Dr. Tom Brenna; Cornell University

Presentation: 

Tobias, H.J.; Ruesch, J.M.; Brenna, J.T. “High Volume Steroid Stable Isotopic Standards for Anti-Doping Applications,” 63^nd ASMS Conference on Mass Spectrometry and Allied Topics, St. Louis MO, May 31-June 4, 2015.

Principle Investigator:
Dr. David Cowan; King’s College London Drug Control Centre

Principle Investigator:
Dr. Detlaf Thieme; IDAS Dresden

Principle Investigator:
Dr. Christoph Borchers; University of Victoria

Publications: 

Molecular and Cellular Proteomics: Multiple Reaction Monitoring Enables Precise Quantification of 97 Proteins in Dried Blood Spots

Presentations: 

Andrew G. Chambers, Andrew J. Percy, Juncong Yang and Christoph H. Borchers, “Highly‐Multiplexed
Protein Quantification from Dried Blood Spots.” 13th Human Proteome Organization World, Madrid,
Spain, October 2014.

Andrew G. Chambers, Andrew J. Percy, Juncong Yang and Christoph H. Borchers, “Quantitative Dried
Blood Spot Proteomics by LC‐MRM‐MS.” 63rd American Society for Mass Spectrometry, St. Louis,
Missouri, USA, May 2015.

Christoph H. Borchers, “MRM and iMALDI for Clinical Research and Diagnostic.” Proteomic Forum Berlin,
Berlin, Germany, March 2015.

Christoph H. Borchers, “Multiplexed Protein Quantitation in Biofluids and Dried Blood Spots by 1D
and/or 2D LC‐MRM/MS for Biomarker Assessment” Agilent Webinar, March 2015.

Principle Investigator:
Dr. James Watkins; Norwich Ventures

Principle Investigator:
Dr. Frank Suhr; German Sport University Cologne

Principle Investigator:
Dr. Daniel Eichner; Sports Medicine Research and Testing Laboratory (SMRTL)

Presentations: 

Cox, HD, Eichner, D. Targeted quantification of blood cell marker proteins in dried blood spots for the detection of blood doping practices in sport. June 2015. 63rd Meeting of the American Society for Mass Spectrometry. St. Louis, MO.

Cox, HD, Eichner, D. Preliminary evaluation of a novel dried blood spot-based methodology for the detection of blood doping practices in sport. September 2014. USADA Science Symposium. Phoenix, AZ.

Cox, HD, Hughes, CM, Eichner, D. Method to detect blood doping practices in sport through the measurement of reticulocytes in dried blood spots by LC-MS/MS. June 2014. 62nd Meeting of the American Society for Mass Spectrometry. Baltimare, MD.

Principle Investigator:
Dr. Mike Ashenden; Science and Industry Against Blood doping (SIAB) Australia

Principle Investigator:
Dr. Jenny Schulze; Karolinska Institutet

Publications:

Frontiers in Endocrinology: The impact of genetics and hormonal contraceptives on the steroid profile in female athletes

Drug Testing and Analysis: Urinary steroid profile in females – the impact of menstrual cycle and emergency contraceptives

Presentation: 

Schulze, J. The impact of CYP17 polymorphims and hormonal contraceptives on the steroid profile in female athletes . 32nd Manfred Donike Cologne Workshop on Dope Analysis. 2014. Cologne, DE.

Principle Investigator:
Dr. Philip Smith; University of North Carolina at Chapel Hill

Presentation: 

Fallon, K, and Smith, PC. Does Interindividual UGT2B17 Expression and Testosterone Glucuronidation Support the Use of the Testosterone/Epitestosterone (T/E) Ratio? 2015. 20th North American ISSX Conference. Orland, FL.

Principle Investigator:
Dr. Barbara Daniel; King’s College London

Presentation: 

Daniel, B. Can Genomic Analysis Be the Answer to Autologous Blood Transfusion Detection?PCC Conference ‘The Doping Decision: Deterring Doping in Sport’.  December 2011, New York.

Principle Investigator:
Dr. Richard Snyder; University of Florida

Publications:

Gene Therapy: Longevity of rAAV Vector and Plasmid DNA in Blood after Intramuscular Injection in Non-Human Primates: Implications for Gene Doping

PLoS One: Development and Utility of an Internal Threshold Control (ITC) Real-Time PCR Assay for Exogenous DNA Detection

Analytical and Bioanalytical Chemistry: PCR-based Detection of Gene Transfer Vectors: Application to Gene Doping Surveillance

Presentations: 

Ni, Y., Le Guiner, C., Penaud-Budloo, M., Moullier, P., and Snyder, R.O. Molecular Detection of rAAV Vector Sequences in Blood.  17th Molecular Virology Workshop, April 23 – 24, 2010.  Daytona Beach, FL.

Ni, Y., Le Guiner, C., Penaud-Budloo, M., Moullier, P., and Snyder, R.O. Molecular Detection of rAAV Genomes in Blood of Non-Human Primates:  Development of a Reliable Blood Test for the Detection of Gene Doping.  European Society of Gene and Cell Therapy Annual Meeting.  October 22-25, 2010.  Milan, Italy

Ni, Y., Le Guiner, C., Penaud-Budloo, M., Moullier, P., and Snyder, R.O. Longevity of rAAV Vector and Plasmid DNA in Blood after Intramuscular Injection in Non-Human Primates.  18th Molecular Virology Workshop, May 6-7, 2011.  Daytona Beach, FL.

Perez, I.C., Le Guiner, C., Moser, D., Simon, P., Moullier, P., and Snyder, R.O. Comparing PCR Conditions That Detect rAAV Harboring the Human Erythropoietin cDNA: Applications for Gene Doping.  Florida Genetics Symposium.  November 28-29, 2012.  Gainesville, FL.

Perez, I.C., Ni, W., Le Guiner, C., Moser, D., Simon, P., Moullier, P., and Snyder, R.O. Comparing PCR Conditions That Detect a rAAV Vector Harboring the Human Erythropoietin cDNA: Applications for Gene Doping.  20th Molecular Virology Workshop, April 26 – 27, 2013.  Daytona Beach, FL.

Perez, I.C., Ni, W., Le Guiner, C., Moser, D., Simon, P., Moullier, P., and Snyder, R.O. Comparing PCR Conditions That Detect rAAV Harboring the Human Erythropoietin cDNA: Applications for Gene Doping.  UF NSF Research Day.  October 29, 2013.  Gainesville, FL.

Perez, I.C., Ni, W., Le Guiner, C., Moser, D., Simon, P., Moullier, P., and Snyder, R.O. Comparing PCR Conditions That Detect rAAV Harboring the Human Erythropoietin cDNA: Applications for Gene Doping.  Florida Genetics Symposium.  October 9-10, 2013.  Gainesville, FL.

Principle Investigator:
Dr. Andy Hoofnagle; University of Washington

Publications: 

Clinical Chemistry: Interlaboratory agreement of insulin-like growth factor 1 concentrations measured by mass spectrometry

Presentations: 

Hoofnagle, A. “Prioritizing Our Validation Efforts.” Proteomics in the Clinic (Hosted by the FDA). Silver Spring, MD. (June 2014)

Hoofnagle, A. “If It’s Broke, Why Not Fix It? How Mass Spectrometry Can Make the Most of Our Precious Samples.” Advancing translational research Using mass spectrometry (Hosted by the Duke Proteomics Core Facility with Support From the Duke Translational Research Institute). Durham, NC. (May 2014)

Hoofnagle, A. “What Contributes to Inter-laboratory Variability of Targeted Protein LC-MS/MS Assays: A Case Study Using IGF-1.” Mass Spectrometry: Applications to the Clinical Laboratory Annual Meeting, San Diego, CA. (March 2014)

Principle Investigator:
Dr. Marijke Grau; German Sport University Cologne

Publications: 

Clinical Hemorheology and Microcirculation: Decrease in red blood cell deformability is associated with a reduction in RBC-NOS activation during storage

Principle Investigator:
Dr. Donald Siegel; University of Pennsylvania

Publications:

Immunologic Research: Translational applications of antibody phage display

Emerging Technologies in Transfusion Medicine: Diagnostic and Therapeutic Applications of Phage Display Technology

Principle Investigator:
Dr. Thomas Piper; Deutsche Sporthochschule Koln

Publications: 

Drug Testing and Analysis: Investigations on hydrogen isotope ratios of endogenous urinary steroids: reference population-based thresholds and proof-of-concept

Analytical and Bioanalytical Chemistry: Effect of changes in the deuterium content of drinking water on the hydrogen isotope ratio of urinary steroids in the context of sports drug testing.

Analytical and Bioanalytical Chemistry: Combination of carbon isotope ratio with hydrogen isotope ratio determinations in sports drug testing

Presentations: 

Manfred Donike Workshop – 30^th Workshop on Dope Analysis, 26.02 to 02.03.2012, Cologne, Germany

Joint European Stable Isotope Users Group Meeting – JESIUM 2012, 2–7 September 2012, Leipzig, Germany

Manfred Donike Workshop – 31st Workshop on Dope Analysis, 24.02 to 01.03.2013, Cologne, Germany

Jahrestagung der Deutschen Gesellschaft für Massenspektrometrie, 10.03 to 13.03.2013, Berlin, Germany

Principle Investigator:
Dr. Jason Dragoo; Leland Stanford Junior University

Publications: 

The American Journal of Sports Medicine: The Systemic Effects of Platelet-Rich Plasma Injection

Presentations: 

Wasterlain AS, Braun HJ, Kim HJ, Dragoo JL. “Systemic Performance-Enhancing Effects of Platelet-Rich Plasma (PRP) Injection.” European Society of Sports Traumatology, Knee Surgery and Arthroscopy (ESSKA) Congress, Geneva, Switzerland. May 4, 2012.

Wasterlain AS, Braun H, Harris A, Kim HJ, Dragoo JL. “Systemic Performance-Enhancing Effects of Platelet-Rich Plasma (PRP) Injection.” American Academy of Orthopaedic Surgeons (AAOS) Congress, Chicago, IL. Mar 19-23, 2013.

Wasterlain AS, Braun HJ, Kim HJ, Dragoo JL. “Systemic Performance-Enhancing Effects of Platelet-Rich Plasma (PRP) Injection.” Arthroscopy Association of North America (AANA) Annual Meeting, Orlando, FL. May 19, 2012.

Wasterlain AS, Braun HJ, Kim HJ, Dragoo JL. “Effect of Platelet-Rich Plasma Injection on Systemic Concentrations of Performance-Enhancing Growth Factors.” Orthopaedic Research Society (ORS) Congress, San Francisco, CA. Feb 6, 2012.

Principle Investigator:
Dr. David Bruns; University of Virginia

Publications:

Clinica Chimica Acta: Quantification of growth hormone mRNA in blood.

Drug Testing and Analysis: Circulating miRNA as a biomarker of human growth hormone administration to patients.

Presentations:

BN Kelly, DM Haverstick, ML Vance, MD Thorner and DE Bruns. Circulating RNAs as markers of growth hormone doping. Growth Hormone and IGF-1 Research Society Meeting, October 2012, MUNICH

Kelly BN, Haverstick DM, Bruns DE. Circulating RNAs as Markers of Growth Hormone Doping. 2012 Meeting. Growth Hormone Research Society. Berlin.

Kelly BN, Haverstick DM, Bruns DE. Circulating RNA as a marker for growth hormone doping. 2012 Academy of Clinical Laboratory Physicians and Scientists (ACLPS) Annual Meeting, Milwaukee.

Kelly BN, Haverstick DM, Bruns DE. Detection of growth hormone mRNA in circulation. 2011 Academy of Clinical Laboratory Physicians and Scientists (ACLPS) Annual Meeting, St. Louis. (Young Investigator Award, Kelly).

Kelly BN, Haverstick DM, Bruns DE. RNA-Biomarker approaches for detection of recombinant human growth hormone abuse. US Antidoping Agency Meeting, London, UK. September, 2011.

Kelly BN, Haverstick DM, Bruns DE. Pathology Research Progress Report meeting;  October, 2011, Charlottesville, VA

Principle Investigator:
Dr. Pauline Rudd; National Institute for Bioprocessing Research and Training

Principle Investigator:
Dr. Andrew Kicman; King’s College London

Principle Investigator:
Dr. Richard Holt; University of Southampton

Publications:

Drug Testing and Analysis: Novel markers to detect recombinant human insulin-like growth factor-I (rhIGF-I)/rhIGF binding protein-3 (rhIGFBP-3) misuse in athletes

Drug Testing and Analysis: Biochemical markers of recombinant human insulin-like growth factor-I (rhIGF-I)/rhIGF binding protein-3 (rhIGFBP-3) misuse in athletes.

BMC Medical Research Methodology: Statistical methodology for age-adjustment of the GH-2000 score detecting growth hormone misuse

Indian Journal of Endocrinology and Metabolism: Detecting growth hormone misuse in athletes

n Athletes

Principle Investigator: 
Dr. Monica Mazzarino;  Labortio anti doping FMSI

Principle Investigator: 
Dr. Daniel Eichner; Sports Medicine Research and Testing Laboratory (SMRTL)

Principle Investigator: 
Dr. Nicolas Leuenberger; Swiss Laboratory for Anti-Doping Analyses

Publications: 

Analytical and Bioanalytical Chemistry: Circulating miRNAs: a new generation of anti-doping biomarkers

PLoS One: Circulating microRNAs as Biomarkers for Detection of Autologous Blood Transfusion

Advances in Experimental Medicine and Biology: Circulating microRNAs : The future of Biomarkers in Anti-doping Field 

Transfusion Medicine Reviews : Autologous Blood Transfusion in Sports : Emerging Biomarkers

Presentations: 

Circulating microRNAs to detect autologous blood transfusion. Presented by: N Leuenberger, YO Schumacher, T Pottgieser, M Saugy and S Pradervand. The Manfred Donike Workshop on Doping Analysis, February 2012, COLOGNE

Principle Investigator: 
Dr. Svetlana Appolonova; Moscow Anti-Doping Laboratory

Presentations: 

Appolonova, S. A., Mesonzhnik N.V. and Rodchenkov, G. M. (2012), Multivariate analysis in combination with electrospray ionization tandem mass spectrometry as a model for prediction of 3-ketosteroids’ and corticosteroids’ structures // Eighth Winter Symposium on Chemometrics “Modern Methods of Data Analysis”. Russia, Drakino, February 27 – March 2, 2012, P. 51-52

Mesonzhnik N.V., Appolonova, S. A., and Rodchenkov, G. M. (2012), Detecting unexpected drugs of abuse using UPLC-HRMS in combination with multivariate analysis // Eighth Winter Symposium on Chemometrics “Modern Methods of Data Analysis”. Russia, Drakino, February 27 – March 2, 2012, P. 52-53

Svetlana Appolonova, Natalia Mesonzhnik, Daria Brazhnikova and Grigory Rodchenkov. PLS-DA and OPLS-DA as a model for prediction of the steroids structures // 29th (MONTREUX) SYMPOSIUM on Liquid Chromatorgaphy/Mass Spectrometry, 7-9 November, 2012, p. 10

Svetlana Appolonova, Natalia Mesonzhnik, Daria Brazhnikova and Grigory Rodchenkov. Validation of multivariate analysis for classification of anabolic steroids and corticosteroids by MS/MS spectra // 29th (MONTREUX) SYMPOSIUM on Liquid Chromatorgaphy/Mass Spectrometry, 7-9 November, 2012, p. 11

Principle Investigator: 
Dr. Andrew Phillips; Yale University

Principle Investigator: 
Dr. Fred Schaufele;  UC San Francisco

Publications: 

PLoS One: Advantages and Limitations of Androgen Receptor-Based Methods for Detecting Anabolic Androgenic Steroid Abuse as Performance Enhancing Drugs

Principle Investigator: 
Dr. Peter Van Eenoo; University of Ghent Doping Control Laboratory 

Publications:

Biomedical Chromatography: In-vitro and in-vivo metabolism studies of dimethazine

Biomedical Chromatography: Metabolism of methylstenbolone studied with human liver microsomes and the uPA⁺/⁺-SCID chimeric mouse model

Presentations: 

Presentation: Geldof L, Lootens L, Deventer K, Polet M, Eichner D, Botrè F, Meuleman P, Leroux-Roels G, Van Eenoo P. In vitro and in vivo metabolism studies of a nutritional supplement containing methylstenbolone. 31st Cologne Workshop on Dope Analysis. 2013. Cologne, Germany.

Lootens L, Geldof L, Meuleman P, Leroux-Roels G, Deventer K, Van Eenoo P. Steroid metabolism studied via a chimeric mouse model in the framework of sports doping. 7th International Symposium Hormone and Veterinary Drug Residue Analysis. June 2014. Ghent, Belgium.

Geldof L, Lootens L, Van Lysebeth J, Meuleman P, Leroux-Roels G, Deventer K, Van Eenoo P. Metabolic study of a steroid product containing dimethazine using human liver microsomes and a chimeric mouse model. 32nd Cologne Workshop on Dope Analysis. 2014. Cologne, Germany.

Principle Investigator: 
Dr. Michael Collins; National Measurement Institute, Australia

Publications: 

Drug Testing and Analysis: Stable carbon isotope ratio profiling of illicit testosterone preparations – domestic and international seizures

Principle Investigator:
Dr. Diana Wilkins; University of Utah

Principle Investigator:
Dr. Doug Rollins; University of Utah

Principle Investigator:
Dr. Anthony Butch; UCLA Olympic Analytical Laboratory

Publications: 

Clinical Chemistry: Immunoextraction – Tandem Mass Spectrometry Method for measuring intact human chorionic gonadotropin, Free ß-subunit, and ß-subunit core fragment in urine

Clinical Biochemistry – Evaluation of a semi-quantitative pregnancy device for susceptibility to interference caused by hCG beta core fragment. 

Drug Testing and Analysis – Urinary human chorionic gonadotropin isoform concentrations in doping control samples.

Clinical Biochemistry – Estimating the hCG beta core fragment in urine during pregnancy.

Presentations: 

Clinical Chemistry Podcast – Immunoextraction – Tandem Mass Spectrometry Method for measuring intact human chorionic gonadotropin, Free ß-subunit, and ß-subunit core fragment in urine.

Cologne Workshop oral presentation – Development of an immunoextraction technique for the quantitation of urinary hCG, hCG free beta and hCG beta core fragment by LC-MS/MS. April 2014.

Cologne Workshop oral presentation – Human chorionic gonadotropin (hCG) isoform concentrations in doping control samples considered negative, positive and atypical. February 2016.

Principle Investigator:
Dr. Vanessa Agon; National Measurement Institute, Australia

Presentations: 

Agon, VW., Goebel, C. Analysis of derivatised carbohydrates of natural and recombinant peptide hormones by LC/MS/MS. 29th Manfred Donike Workshop. February 2011. Cologne, Germany.

Principle Investigator: 
Dr. Tom Brenna; Cornell University

Publications: 

Analytical Chemistry: Detection of synthetic testosterone use by novel comprehensive two-dimensional gas chromatography combustion-isotope ratio mass spectrometry

Drug Testing and Analysis: Comprehensive 2-dimensional gas chromatography fast quadrupole mass spectrometry (GC × GC-qMS) for urinary steroid profiling: Mass spectral characteristics with chemical ionization

Analyst:  Highly sensitive and selective analysis of urinary steroids by comprehensive two-dimensional gas chromatography combined with positive chemical ionizationquadrupole mass spectrometry

Presentations: 

Herbert Tobias; Joseph Ruesch; J Thomas Brenna. High Volume Steroid Stable Isotopic Standards for Anti-Doping Applications. American Society for Mass Sspectrometry Annual Conference on Mass Spectrometry and ALlied Topics, St Louis, Mo, June 2015.

J Thomas Brenna. Translational Research: High Volume Steroid Stable Isotopic Standards for Anti-Doping Applications. Partnership for Clean Competition 2017 Conference. New York, NY, 2017.