Francesco Botrè, PhD, is Director of the WADA-Accredited Laboratorio Antidoping Federazione Medico Sportiva Italiana (FMSI Anti-Doping Lab). He shares the history of FMSI Lab and the complex process of how samples are collected, delivered, anonymized, processed, analyzed, and interpreted by the lab. Francesco also discusses his research on doping masking agents, metabolic modulators, and drug-drug interactions, as well as his experiences running testing in the 2006 Winter Olympic Games in Torino, Italy.